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Breast cancer (BC) represents the most common cancer in women and is one of the leading causes of cancer-related deaths among them. An increment in total hexosaminidase (Hex) levels has been described in patients with BC. Consequently, Hex activity is increased in BC. However, Hex specificities have not been studied thoroughly to determine the cell type where Hex is over-expressed. In this study, we evaluated Hex activities in blood and in lipid-rich fractions from BC patients and healthy controls. We also evaluated the Hex activity in HepG2 and MCF7. Blood and lipid fractions were obtained by centrifugation of untreated plasma (900×g for 15 min). BC tissue samples (n = 26) and adjacent normal tissues (n = 28) were obtained from the archives of the Breast Surgery and Oncology Center, St. Panteleimon, Thessaloniki, Greece. The study was conducted in accordance with the Declaration of Helsinki and was approved by the Ethical Committee of the National and Kapodistrian University of Athens, Athens, Greece. Lactate dehydrogenase (LDH) was used as an internal control for leukocyte contamination. Hex was evaluated with the artificial substrate 4-methylumbelliferyl-α-D-N-acetylglucosamine and with the naturally occurring substrate 4-methylumbelliferyl-α-D-N-acetylneuraminic acid. In the cell culture experiments, we used HepG2 and MCF7 cell lines to evaluate Hex, α-neuraminidase and β-galactosidase activities and
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