Sims 4 Reptile Skin
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Sims 4 Reptile Skin
Females were chosen to ensure that the samples could be compared across treatments. DIBP was not detectable in samples from any reptile. DNOP was detectable in control and treatment 1 female iguana.
Recent investigations have explored the relationship between reptile size and the relative abundance of phthalates in the environment [2]. We found the presence of phthalates in non-exposed animals and quantifiable levels in non-exposed reptiles. Smaller lizards had significantly higher concentrations of all three phthalates in the liver and adipose tissues. Greater exposure (e.g., greater surface area and/or greater metabolic demands) is a likely explanation. It is hypothesized that natural selection may lead to evolutionary shifts in reptile morphology or physiology that enable them to better extract phthalates from the environment. Larger snakes may have a reduced ability to bioaccumulate phthalates due to increased metabolic activity, limiting the ability of phthalates to cross the blood brain barrier. Consequently, the association of temperature as a method to control phthalate concentrations may be more effective in larger reptiles. It is important to note that although the assay was optimized for detection, it is possible that the difference in detection of phthalates in the smaller individuals was not enough to statistically detect a difference due to the small sample size. Further investigation is needed to assess whether size differences in reptiles are associated with increased, decreased, or equal phthalate concentrations.
Phthalates were detected in all ventral skin samples at concentrations slightly above (median) those found in other tissues. Extraction of ventral skin was performed as a single sample, encompassing the entire dosed area. It is possible that one or more phthalates were sequentially transferred through the skin to cause a statistical bias. An alternative explanation would be that contaminants that remained on the skin throughout the course of the experiment exceeded our detection limits. A more thorough distribution across the skin and less passive movement may produce these results. However, neither explanation would account for the presence of phthalates in oral dosed samples.
In the same way we are classifying them based on the similarities of their external features, amphibians are also easy to identify. Amphibians are a unique group of animals that share several traits. They are also cold-blooded (ectothermic) animals. They shed their skin once a year, and they have gills, a kidney, and an anus. Amphibians have a lung that houses the air sac and is not covered by the skin. The kidney is located in the same place as ours, and the common amniotic sac, or the amnion, is another, separate place where the amniotic fluid is stored. In fact, the amniotic fluid is also used to protect the fetus. That same fluid allows many to breath with their skin. While most amphibians can breathe with their skin, many are more specialized and only need it when it is wet. While not unique, amphibians are also found in many different parts of the world, and some live in more extreme environments than others. One of the most amazing things about amphibians is that they can both control their body temperature and create their own warmth. This allows them to live in both tropical and temperate areas. One fun thing that is similar about amphibians is that they do not hibernate.
Two SRMs (0505 and 0702, Standard Reference Materials) were used for method validation. Since no isotope-dilution studies are available for reptile skin and fat, we used the isotope dilution method of [18] (a method initially developed for fish muscle). A range of calibration standards was prepared by spiking SRMs onto blank samples (methanol only) to a calibration concentration range of 0.5-60 ng/g. All samples of the study were spiked on three occasions. Extracts were transferred to a screw-top microtiter plate and evaporated to dryness. Extracts were reconstituted in an appropriate diluent, evaporated to dryness and a 100 L aliquot of a 100 g/mL deuterated surrogate (d4 deuterated di-ethyl phthalate) was added. Extracts were concentrated to 1 mL by nitrogen evaporation. D4 DEP was separated from other coextracts using a solid-phase extraction column (Supelclean LC-Si NH2, Supelco) according to manufacturer recommendations and eluted into GC-MS glass vials. Extracts of the SRMs were pooled, spiked with surrogates, and run on the GC-MS to obtain the ion ratios. For the quantification studies, skin samples were first extracted with MeCl2 (8 mL per 2 g). They were then spiked with 30 ng/g of ^13^C-d4 DEP and extracted. An aliquot (4 mL) was spiked with d4 DEP and the pooled skin extracts were evaporated to dryness and reconstituted in a suitable diluent for analysis. All analyses were performed in positive-ion electron capture mode with a 6890N GC-5975 MSD (Agilent Technologies, Palo Alto, CA). Extracts were injected onto a 30 m CNW (5% phenyl)- polydimethylsiloxane column (30 m×0.25 mm I.D., coating, 0.1 μm; Restek Corporation, Bellefonte, PA), at 40°C for 6 minutes and ramped to 300°C at 8°C/minute. Quality assurance and quality control were performed by the National Institute of Standards and Technology, Columbus, OH. Intercomparison studies were performed by the National Metrology Institute of Japan, Tokyo, Japan. Experimental data were analyzed using the ChemStation software, Rev. B.04.01 (Agilent Technologies, Santa Clara, CA). All procedural blanks, calibrators, and quality control samples were below the reporting limits and were assigned an analytical value of zero.
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